Objective: To assess the perspectives and preferences of medical students and residents regarding professionalism and ethics education. Methods: A new written survey with 124 items (scale: "strongly disagree" = 1, "strongly agree" = 9) was sent to all medical students (n = 308) and PGY 1-3 residents (n = 233) at one academic center. Results: Of the 336 participants (200 students, 65% response; 136 residents 58% response), only 18% found current professionalism and ethics preparation sufficient. Respondents endorsed professionalism (means = 7.48 to 8.11) and ethics topics (means = 6.56 to 6.87), women more so than men (p less than 0.05). Respondents preferred clinically- and expert-oriented learning over formal, nontraditional, or independent approaches (p less than 0.0001). They preferred clinically-oriented assessment methods (p less than 0.0001), residents more so than medical students (p less than 0.0001). On several items, psychiatry residents expressed greater receptiveness to professionalism and ethics preparation. Conclusions: Medical students and residents indicate support for professionalism and ethics educational initiatives, including diverse curricular topics and clinically-attuned assessments.