The advanced interdisciplinary research laboratory (AIRLab) represents a novel, effective, and motivational course designed from the interdisciplinary research interests of chemistry, physics, biology, and education development faculty members as an alternative to the independent thesis project experience. Student teams are assembled to work toward the completion of an interdisciplinary research project. Each team is composed of at least one student from a differing area of specialization (e.g., biology, biotechnology, chemistry, and physics), and projects are based on current trends in research. The inaugural project was the development of a portable DNA sequencer for in-field species identification; a project at the interface between physical and life sciences and that holds the potential for real-world application and positive social change. This work describes the details underlying the design and implementation of the AIRLab course, and includes an account of the method of student team assembly, the selection of a suitable research challenge, the specialized training provided in Agile project management, the methods used to achieve cohesive team dynamics, the learning outcomes from this experience, future directions that will be pursued for course improvement, and how our undergraduate degree-level expectations were met at an advanced level.