Chemical separations are an important part of an undergraduate chemistry curriculum. Sophomore students often get experience with liquid-liquid extraction in organic chemistry classes, but liquid-liquid extraction is not as often introduced as a quantitative sample preparation method in honors general chemistry or quantitative analysis classes. This experiment offers a relevant, interesting way to use liquid-liquid extraction and high-performance liquid chromatography (HPLC) to analyze popular lotions and creams for niacinamide content. After completing the experiment, students will have a better understanding of the extraction technique, Beer-Lambert law, and HPLC. The percent recovery from a spiked sample of Olay Original Active Hydrating Beauty Fluid was found to be 99.22% with a standard deviation of 0.01%.