The present study aimed at adapting in the Greek language the Students' Motivation Towards Science Learning (SMTSL) questionnaire developed by Tuan, Chin, and Shieh ("INT J SCI EDUC" 27(6): 639-654, 2005a) into a different cultural context, a different age group, that is, in university students and with a focus on physics learning. Three hundred and fifty Greek student teachers participated in the study. The original instrument consisted of 35 items allocated in six scales: self-efficacy, use of active learning strategies, science learning value, performance goals, achievement goals, and learning environment stimulation. The instrument's internal consistency was acceptable and comparable to previous studies' reports. Confirmatory factor analysis (CFA) was applied on the data in order to test an a priori hypothesis regarding the SMTSL's factorial structure based on previous studies' findings. The results of the study showed that the six-factor conceptual model of students' motivation proposed by the SMTSL applies in this different cultural setting and in this group of university students with reference to physics learning. Along with the six distinct motivational constructs confirmed, students' motivational beliefs were also explained by a general motivational construct assumed to be at their basis. Suggestions for further improvement of the Greek version of the SMTSL are also discussed.