A wide range of activities may be undertaken in elementary and secondary school science laboratories as part of regular curricular activities or optional classroom activities, including science fair projects. Among these is the culturing of microorganisms such as bacteria or fungi. There are various potential educational opportunities associated with these activities, however there are, as well, inherent biosafety concerns associated with culturing of certain materials or microorganisms. In diagnostic and research laboratories, strict biosafety protocols are mandated to reduce the risk of laboratory-associated infections. Currently, the author is unaware of any objective information regarding culture practices used in school classrooms and whether there are any potential health and safety risks that are not being adequately addressed. Further, specific guidelines for bacterial or fungal culture in school laboratories appear to be either superficial or lacking, and are not based on evidence regarding current practices. This study characterizes bacterial and fungal culture practices in classrooms in schools. (Contains 4 tables.)