Objective and Participants: The authors of this retrospective case study focused on antimicrobial susceptibility of urinary tract isolates in cases of acute uncomplicated cystitis in college-aged women, aged 18 to 24 years, diagnosed at a major south-central university student health center. Methods: The authors obtained pertinent data from patient medical and laboratory records, including, diagnoses, urine culture, and susceptibility results. Results: Of 179 isolates, 161 (90%) were "Escherichia coli" ("E. Coli") isolates, of which 23% were resistant to trimethoprimsulfamethoxazole, less than 1% were resistant to Ciprofloxacin, and less than 1% were resistant to nitrofurantoin. Sixty-eight (42%) "E. Coli" isolates were multidrug resistant. There were 18 isolates cultured in the study group that were significant organisms other than "E. coli." For all organisms in the study group, there was a combined resistance rate of 22% to trimethoprim-sulfamethoxazole, less than 1.0% for Ciprofloxacin, and 7% for nitrofurantoin. Conclusions: The findings suggest that both Ciprofloxacin and Nitrofurantoin are acceptable choices for empiric therapy of acute uncomplicated urinary tract infection in this study population, keeping in mind the potential for rising resistance rates to Ciprofloxacin in the community setting.