Objective: Determine if undergraduate athletic training students enrolled in an accredited athletic training education program (ATEP) and participating in clinical assignments experience burnout. Design and Setting: Undergraduate athletic training students enrolled in a clinical education course were surveyed during the fourth and twelfth weeks of a 15 week semester. Participants answered eight demographic/status questions for correlation. Subjects: Fifty-one undergraduate athletic training students in a Commission on Accreditation of Athletic Training Education (CAATE) accredited ATEP participated. Measurements: Data were analyzed using covariance analysis with categorical independent variables and continuous covariants. Emotional exhaustion (EE), depersonalization (DP), and personal accomplishment (PA) were scored in accordance with the Maslach Burnout Inventory-Human Services Survey (MBI-HSS). Post hoc testing was done when interactions/effects were significant at p = 0.05. Dependent variables included EE, DP, and PA. Results: Overall mean scores demonstrated moderate levels of EE (17.0), low levels of DP (5.7), and moderate levels of PA (38.0). Fourth (+) semester students (students in their 4th or 5th semester of the ATEP) reported high DP levels (13.3) and high levels of EE (30.9). Semester effect (p = pre- 0.0001; post- 0.007) and semester by gender interaction (p = pre- 0.0017; post- 0.005) had an effect on EE for both testing times. For DP, semester (p = pre- 0.0001; post- 0.0003), and semester by gender interaction (p = pre- 0.0001; post- 0.0001) were significant interactions/effects for both testing periods. Conclusions: Undergraduate athletic training students demonstrated a moderate degree of burnout from their clinical assignments and associated responsibilities. The ATEP appeared to have a cumulative effect evidenced in the fourth (+) semester with a high/average degree of burnout.