The purpose of this study was to determine if changes in positive well-being, psychological distress, fatigue, and enjoyment vary as a function of physical activity mode. Fifty-five senior high school students participated in one of four fitness activities including two defined as traditional (running and step-aerobics) and two defined as sport-related (ballistic basketball and ultimate indoor soccer). Participants engaged in the activities every other day at moderate exercise intensity for approximately 20 minutes. Measures of acute feeling states were obtained immediately prior to and following participation in each activity while enjoyment was assessed post-activity only. Participants reported increases in positive well-being and psychological distress and reductions in fatigue following acute exercise, regardless of activity mode. No significant differences were found as a function of activity mode for positive well-being, psychological distress, or fatigue. A significant main effect for activity mode was found, however, for post-activity perceptions of enjoyment. Specifically, participants enjoyed sport fitness activities more than traditional fitness activities. The results of this study suggest that physical education students experience (a) greater enjoyment from sport fitness activities than they do from traditional fitness activities, and (b) both traditional and sport fitness activities are associated with improvements in positive well-being and fatigue over time when performed at moderate exercise intensity. (Contains 1 figure and 4 tables.)