The study is focusing on development of an instrument to determine science-specific epistemological beliefs of prospective science teachers. The study involved 364 (male = 82, female = 282) prospective science teachers enrolled in a science teacher education program. The confirmatory factor analysis, reliability analysis and correlation analysis were done for validating 15-item instrument. The confirmatory factor analysis results confirmed that the instrument had 5 dimensions (speed of knowledge acquisition, tentativeness of knowledge, structure of knowledge, source of knowledge and control over knowledge acquisition). The Cronbach alpha values of all dimensions ranged from 0.42 to 0.60. The correlation analysis showed that dimensions were partially independent from each other. The results of the study supported validation of a five-dimension instrument with partially independent dimensions except for low reliability values.