Object individuation was investigated in newborn domestic chicks. Chicks' spontaneous tendency to approach the larger group of familiar objects was exploited in a series of five experiments. In the first experiment newborn chicks were reared for 3 days with objects differing in either colour, shape or size. At test, each chick was presented with two groups of events: two objects differing in one property vs. two presentations of the same object. In both cases, all objects involved in the same group of events were sequentially presented and eventually concealed in a different spatial location, and the number of events taking place at each location was equalized. Chicks spontaneously approached the two different objects rather than the single object seen twice. Chicks did not just prefer the more varied set as they did not choose it when the two elements of each group of events were simultaneously presented (Experiment 2). Chicks succeeded when two different objects simultaneously presented were confronted with three identical ones simultaneously presented (Experiment 3), though they failed with sequential presentation of two different objects vs. one object presented three times if they had been familiarized with up to three identical objects (Experiment 4). Chicks instead succeeded if they had been familiarized with objects that were all different from one another (Experiment 5). These young birds thus proved able to use property and spatiotemporal information for object individuation.