In business education advanced collaboration skills and media literacy are important for surviving in a globalized business where virtual communication between enterprises is part of the day-by-day business. To transform these global working situations into higher education, a learning scenario between two universities in Germany and Austria was created where students worked together in virtual interregional learning groups. This article reports about a study of an interuniversity collaborative learning scenario within the subject of e-business. Participating students collaborated virtually and documented a shared case study in a Wiki. When working together, learners used different synchronous and asynchronous tools for close virtual collaboration around a Wiki toolset such as forum, chat, video conferencing and other social media. Students applied given case studies (e.g. from "Harvard Business Review") or they worked out a business case from their own experience, which covered a range of upcoming e-business topics. In an attending evaluation study with around 460 participants from two universities, 259 questionnaires were evaluated. It reveals several substantive effects like: (1) Tremendous influence of interregional group work for media competencies; (2) Hidden social aspects and conflict potential; (3) Scenario design and different media usage; (4) Teaching effort vs. learning outcome of such a scenario; and (5) Learning impact for different student groups depending on gender, employment, graduation or online-moderation. The findings of this study reveal several interesting aspects concerning media usage and show how students benefited from Wiki work in this virtual learning scenario.