Research has demonstrated that an important factor in the success of inclusive education is dependent upon teachers' attitudes. Based on this evidence, the present study investigated the impact of a range of teacher variables in association with training on primary pre-service teachers' attitudes by examining total inclusion scores, positive affect, training and perceived competence, and negative affect. Three-hundred and forty-two participants from a metropolitan Australian University studying primary school education completed the Teacher Attitudes to Inclusion Scale (adapted). Comparisons were made between participants who had received training on special education, compared with those who had not. The analyses revealed that overall, studying an elective unit on inclusive education had a positive influence over pre-service teachers' attitudes. It was found that teaching experience had a significantly negative impact on pre-service teachers' attitudes. The present study demonstrates the vital role that training institutions play in developing appropriate attitudes to inclusive education with significant issues around teacher training being examined. The research has important implications for training institutions, with future research being discussed.