Behavioral measures, fractionated reaction and reflex times by means of electromyography, were used to determine if the eye color differences are found in the central or peripheral regions of the nervous system. The purpose of this research was to determine the truth of the hypothesis that dark-eyed individuals have faster reflex and reaction time than those with light eyes. Two experiments were conducted on subjects divided equally by eye pigmentation. The first fractionated simple reaction time into premotor and motor components by electromyography. Analysis of variance revealed that dark-eyed subjects had faster total reaction times and premotor times, but only the premotor time component approached significance. The second experiment fractionated patellar reflex time of light-eyed and dark-eyed subjects into reflex latency and motor components. There were no eye color differences for any of the reflex-time measures. The results of the two experiments support a central nervous system explanation for the iris pigmentation-reaction time phenomenon. (Authors/JD)